概要：The ARPE-19 cell line is spontaneously immortalized retinal pigment epithelia (RPE) cell line derived from a 19-year-old male.
ARPE-19 cells exhibit structural and functional properties that are characteristic of RPE cells in vivo.
They are adherent cells and can be cultivated for over 30 passages.
The cells show morphological polarization when plated on laminin-coated filters in media with low serum concentration and form tight junctions with transepithelial resistance of monolayers.
ARPE-19 cells express RPE-specific markers CRALBP and RPE-65, indicating that they can form stable monolayers with morphological and functional polarity.
Compared to other RPE cell lines, ARPE-19 cells have been found to lag on TEER and RPE signature gene expression.
iPSC-RPE, in particular, expresses genes associated with chromatin regulation at an elevated level.
Moreover, they express networks involved in early eye development and muscle contraction.
The cells have a doubling time of approximately 55-65 hours and can undergo 48 population doublings.
These cells are suitable for transfection studies and can be used to model the retinal pigment epithelium pigmentation.
However, it is essential to exercise caution when using ARPE-19 cells to model native RPE cells for the study of retinal diseases due to the presence of both mortal and immortal cells in the cell line.
