83-5600-95　［取扱停止］SiHa Cells 1vial　305023

特徴

• 概要：SiHa cells are a human cervical squamous cell carcinoma cell line that has been widely used in research for several decades.
• They were isolated from primary uterine biopsy fragments from a 55-year-old female Japanese patient with squamous cell carcinoma.
• This cell line is of great interest to researchers studying cervical cancer and other related diseases due to their unique genetic characteristics.
• SiHa cells have been found to express the p53+ and pRB+ genes, which are involved in cell cycle regulation, DNA repair, and tumor suppression.
• These genes make SiHa cells an ideal model for studying the molecular mechanisms of cancer development and progression.
• Additionally, SiHa cells are a suitable transfection host, making them an excellent tool for gene expression studies.
• SiHa cells have a hypertriploid karyotype, with an average chromosome number between 69 and 72.
• The SiHa cells are HPV-16 positive, showing integration of 1 to 2 copies of the viral genome per cell.
• Cells are tumorigenic, forming poorly differentiated epidermoid carcinoma (grade III) in nude mice.
• This makes them an excellent model for studying cancer progression and testing anti-cancer drugs.
• SiHa cell line expresses various isoenzymes, including AK-1, ES-D, G6PD, GLO-I, Me-2, PGM1, and PGM3.
• Electron microscopy revealed abundant tonofilaments in the cytoplasm and desmosomes at the cell junctions.
• The growth properties of SiHa cells are adherent, with a doubling time of 17 hours in 10% FBS media and 21 hours in 5% FBS media.
• Epithelial cell adhesion molecule (EpCAM) expression is present in 92% of SiHa cells, indicating their epithelial origin.
• They show strong cytokeratin expression but no vimentin expression.