概要:This clonal stable cell line was generated by transfection of a circular plasmid followed by drug resistance selection.
The HeLa Kyoto Mad2-LAP/H2B-mCherry cells, which were created by integrating a plasmid containing the tagged-target gene sequence in a non-endogenous locus, may experience variegation or heterogeneity when propagated in culture for extended periods.
Heterogeneity over time may be a plausible outcome.
To mitigate this issue, selective growth of these cells is advised, and if heterogeneity persists, using FACS to select the labelled population can improve cell quality.
仕様
容量:1vial
カテゴリー:Reporter-labeled cells
Organism:Human
Tissue:Cervix
Disease:Carcinoma
Synonyms:HeLa Kyoto Mad2-LAP and H2B-mCherry, HeLa Kyoto Mad2-LAP
Age:30 years
Gender:Female
Ethnicity:African American
Morphology:Epithelial-like cells with mosaic stone shape
Growth Properties:Monolayer, adherent
Citation:HK Mad2-LAP/H2B-mCherry (Cytion catalog number 300920)