概要：The stable clonal cell line HeLa Kyoto EGFP-H2B offers invaluable insights into essential nuclear processes.
Chromatin structure plays a pivotal role in numerous biological phenomena, and the nucleosome, composed of four core histone proteins (H2A, H2B, H3, and H4), is its fundamental building block.
This HeLa derivate expresses green fluorescent protein fused with histone H2B (H2B-GFP), enabling the study of the dynamic architecture of chromosomes during various cellular processes.
By monitoring the behaviour of histone H2B-GFP, insights into the clustering patterns of double minute chromosomes (DMs) in cancer cells during mitosis were gained.
This distinct clustering behaviour contributes to the asymmetric distribution of DMs to daughter cells, shedding light on the complex nature of cancer cell division.
Furthermore, the HeLa Kyoto EGFP-H2B cells provide a unique platform for continuous analysis of chromosomal degradation during apoptosis.
The generation of this cell line involved transfection of a circular plasmid followed by meticulous drug resistance selection.
仕様
容量：1vial
カテゴリー：Reporter-labeled cells
Organism：Human
Tissue：Cervix
Disease：Carcinoma
Synonyms：HeLa Kyoto H2B-EGFP, HeLa Kyoto H2B EGFP, HeLa-H2B-GFP
Age：30 years
Gender：Female
Ethnicity：African American
Morphology：Epithelial-like cells with mosaic stone shape
Growth Properties：Monolayer, adherent
Citation：HK EGFP-H2B (Cytion catalog number 300673)
