83-5600-23　C6 Cells 1vial　500142

特徴

• 概要：The C6 cell line maintains glial cell type with fibroblast morphology and originates from a glioma of a Wisthar-Furth rat.
• The glioma was induced by exposure to N-nitrosomethylurea, following numerous cycles of alternating culture and animal passages.
• The C6 glioma cell line is frequently utilized in neuro-oncology research to create animal models that closely mimic the characteristics of human glioma, aiding in the development of new therapeutic agents and strategies.
• It is particularly effective in 3D cell culture and high-throughput screening.
• C6 cells are genetically diverse, possessing a wild-type p53 gene, increased Rb gene expression, and a mutant p16/Cdkn2a/Ink4a locus but lacking p16 and p19ARF mRNA expression.
• They also overexpress several genes in human gliomas, such as PDGF?, IGF-1, EGFR, and Erb3/Her3 precursor proteins.
• However, the expression of IGF-2, FGF-9, and FGF-10 is reduced, while MMP-7 gene expression remains unchanged.
• Like human gliomas, C6 cells show increased activity of the Ras pathway genes, which is regulated by the elevated expression of the Ras guanine triphosphate activator protein.
• The C6 cell line has been utilized in various studies.
• For instance, it was used to examine the ability of 2-(2,4-dihydroxy phenyl)thieno-1,3-thiazin-4-one (BChTT) to halt cancer cell proliferation and to investigate the mechanisms involved in this process.
• In another research, the cytotoxic and antioxidant properties of the supercritical CO2 extract (SCE) of an old mans beard (Usnea barbata) were studied using C6 cells.
• Interestingly, these cells have been reported to show increased levels of glyceryl phosphate dehydrogenase activity in response to glucocorticoids.