83-5599-86　AR42J Cells 1vial　500478

特徴

• 概要：AR42J cells were derived from malignant nodules in rats pancreas induced with azaserine, resulting in tumour formation.
• Unlike normal acinar cells, AR42J cells exhibit distinct characteristics that differentiate them in several ways.
• Firstly, they display rapid proliferation, allowing for efficient cell culture and replication.
• Secondly, their exocrine function regulation is abnormal due to atypical receptors, such as cholecystokinin octapeptide type B and pituitary adenylate cyclase-activating polypeptide type I receptors.
• This abnormal regulation affects inositol phosphate metabolism and causes disorganization of the cytoskeleton.
• AR42J cells also possess a unique neuroendocrine-regulated pathway, which involves voltage-sensitive ionic currents, post-translational processing of peptidic prohormones, and the release of small neurotransmitters such as gamma-aminobutyric acid, glycine, and glutamic acid.
• This pathway indicates the presence of neuroendocrine characteristics in these cells, further distinguishing them from normal acinar cells.
• These amacrine cells can be seen as a cancerous version of the primordial pancreatic ductular epithelium, as they exhibit characteristics of both exocrine and neuroendocrine cells.
• When treated with dexamethasone, AR42J cells undergo differentiation towards the exocrine phenotype, further highlighting their ability to adapt and respond to external factors.
• In terms of their growth and proliferation, AR42J cells show a preference for specific signalling pathways.
• Occupancy of receptor tyrosine kinases, adenosine 3,5-cyclic monophosphate, ornithine decarboxylase expression, and Na(+)-H+ exchange is factors that contribute to the mitogenic pathway of these cells, promoting their growth and division.
• On the other hand, somatostatin, a hormone that regulates various physiological processes, opposes the proliferation of AR42J cells through protein phosphatases.
• This inhibitory effect helps to maintain cellular homeostasis and prevent excessive cell growth.
• Given their unique characteristics and biological behaviour, AR42J cells are valuable for studying various pancreatic biology and cancer research aspects.
• They serve as a helpful model for investigating the underlying mechanisms of pancreatic tumours and exploring potential therapeutic approaches.
• Additionally, these cells are commonly utilized in 3D cell culture systems, enabling researchers to study cell behaviour and interactions within a more physiologically relevant environment.
• Overall, the AR42J cell line offers a powerful tool for advancing our understanding of pancreatic diseases and developing novel treatments.