83-5599-80　［取扱停止］H9c2（2-1） Cells 1vial　305203

特徴

• 概要：Introducing H9c2(2-1) Cells: A Versatile Cell Model for Cardiomyocyte Research H9c2(2-1) cells are a specialized subclone derived from the original clonal cell line obtained from embryonic BD1x rat heart tissue.
• These cells exhibit remarkable properties similar to skeletal muscle cells, making them an ideal model for studying cardiac muscle function.
• When stimulated by acetylcholine, the myoblastic cells in the H9c2(2-1) cell line can fuse and form multinucleated myotubes.
• These cells are derived from the embryonic heart tissue of the Rattus norvegicus (Rat) species, specifically the cardiac muscle.
• As an alternative to primary cardiomyocytes, H9c2 cells offer a valuable model for cardiac research.
• By reducing the serum concentration in the cell culture media and introducing all-trans-retinoic acid (RA), the H9c2 cells can be induced to differentiate into a cardiac-like phenotype.
• This differentiation process results in multinucleated cells with reduced proliferative capacity, resembling cardiomyocytes.
• In a groundbreaking study, the transcriptional analysis of H9c2 cells was conducted in two differentiation states: embryonic cells and differentiated cardiac-like cells.
• The findings revealed significant upregulation of genes encoding cardiac sarcomeric proteins, calcium transporters, and associated machinery.
• Notably, genes associated with mitochondrial energy production, such as respiratory chain complexes subunits, mitochondrial creatine kinase, carnitine palmitoyltransferase I, and uncoupling proteins, were also observed to increase expression during differentiation.
• Undifferentiated H9c2 myoblasts demonstrated increased expression of genes related to pro-survival proteins and cell cycle regulation.
• The differentiation of H9c2 cells led to the remodelling of mitochondrial function and increased transcripts and protein levels associated with calcium handling, glycolytic and mitochondrial metabolism.
• The involvement of PI3K, PDK1, and p-CREB in H9c2 differentiation was also identified.
• Complex analysis of differently expressed transcripts further revealed significant upregulation of genes associated with cardiac muscle contraction, dilated cardiomyopathy, and other pathways specific to cardiac tissue.
• The H9c2 (2?1) cell line offers several advantages over primary cardiomyocytes.
• Primary cardiomyocytes are delicate and challenging to maintain in culture for extended periods.
• Moreover, their isolation requires the sacrifice of laboratory animals, raising ethical concerns.
• In contrast, H9c2 cells provide a reliable in vitro model for studying cardiac and skeletal muscle, enabling cell biology, electrophysiology, and toxicology research without the need for animal sacrifice.
• Initially isolated from the ventricular part of a BDIx rat heart, the H9c2 cell line undergoes selective serial passages, allowing the separation of different components based on their adhesion kinetics.
• Although not fully differentiated into adult cardiomyocytes, these cells exhibit cardiomyocyte-specific markers, demonstrating their potential for cardiac research.
• By culturing H9c2 cells in a low serum concentration media, they can differentiate from mono-nucleated myoblasts to myotubes, assuming an elongated shape and parallel positioning.
• The addition of all-trans retinoic acid (RA) to the media further induces the presence of cells with an adult cardiac muscle phenotype characterized by the overexpression of the alpha-1 subunit of L-type calcium channels.
• It is important to note that H9c2 cells do not possess contractile activity, even in their differentiated state.
• Nevertheless, these cells respond similarly to various stimuli as isolated neonatal cardiomyocytes, including developing hypertrophic responses.
• However, it is crucial to consider the differentiation between state