概要：The 9L/lacZ cell line, derived from glial cells of Rattus norvegicus (rats), displayed fibroblast morphology and originated from the 9L cell line, a rat gliosarcoma cell line induced by nitrosourea.
Developed in 1989, the 9L/lacZ cell line was created by infecting 9L cells with the BAG replication-deficient retroviral vector carrying the E.
coli lacZ gene encoding beta-galactosidase (beta-gal) and the Tn5 neomycin gene, which imparts resistance to G418.
Notably, the 9L/lacZ cell line is marked by its reporter-labelled attribute, as it expresses the lacZ reporter gene.
This reporter gene enables visualization of beta-gal through histochemical staining, facilitating the identification of individual tumour cells and improving image analysis.
Widely utilized in biological research, the 9L/lacZ cell line finds applications in 3D cell culture, assay development, high-throughput screening, and neuroscience studies.
It serves as a valuable model for investigating brain tumours, closely resembling human tumour growth, allowing for quantitative analysis of microscopic tumours.
These adherent cells possess tumorigenic properties and can generate tumours in CD Fischer 344 rat brains, making them an excellent gliosarcoma model.
While beta-gal expression remains stable, re-cloning may be necessary for long-term maintenance and consistent experimental outcomes.
