83-5599-69　LNCaP Cells 1vial　300265

特徴

• 概要：LNCaP cells are a type of prostate cancer cell line that was derived from a metastatic lymph node lesion of a human prostate cancer patient.
• These cells are notable for expressing the androgen receptor (AR) and exhibiting androgen-sensitive growth, which makes them a valuable model for studying the role of androgens in prostate cancer.
• One of the obstacles in conducting clinically relevant prostate cancer research has been the lack of cell lines that closely mimic human disease progression.
• LNCaP sublines have been generated to provide the most clinically relevant tissue culture tools to address this issue.
• These cells form osteoblastic lesions, which more closely mimic the behavior of human prostate cancer in bone.
• The LNCaP cell line was reported to form subcutaneous tumors in male mice and is highly resistant to human fibroblast interferon.
• These cells have been further characterized and found to be aneuploid with a modal chromosome number of 76 to 91, but wild-type for TP53, and expressing a mutated form of the AR gene, which has broader steroid-binding specificity than the wild-type AR.
• Additionally, LNCaP cells express downstream targets of AR activity, including prostate-specific antigen (PSA), a well-known marker of prostate cancer, and NKx3.1.
• This homeoprotein has essential functions in prostatic epithelial cells.
• The LNCaP cell line is considered one of the classic and most widely used human prostate cancer cell lines, alongside DU145 and PC3 cells.
• Regarding culture, LNCaP cells can be cultured in EMEM media supplemented with 10% FBS.
• The cells grow as a loosely adherent layer and are prone to develop in clusters.
• It is essential not to plate them too sparsely, as this will affect their growth.
• The doubling time for these cells is between 48-60 hours.
• To harvest the cells, they should be digested with 0.25% trypsin-EDTA for 3-5 minutes at 37 degree Celsius or detached using Accutase.
• LNCaP cells are medium-sized cells with an average size of 18 micrometer.
• In terms of seeding density, a guide density of 1 to 2 x 10^4 cells/cm^2 is recommended.
• The cells should be passaged when they reach 2 x 10^5 cm^2.
• In conclusion, LNCaP cells are an essential cell line in prostate cancer studies, especially in the context of androgen-sensitive growth.
• These cells are easy to culture, have a moderate size, and mimic human disease progression more closely than other available cell lines.
• They offer valuable insights into prostate cancers biology and the androgens role in the disease.