概要：The LNCaP clone FGC cell line is a prominent epithelial cell line derived in 1977 by J.S.
Horoszewicz and colleagues from a metastatic prostate carcinoma in a 50-year-old Caucasian male patient.
This cell line originates from a needle aspiration biopsy of the left supraclavicular lymph node, presenting a hypotetraploid karyotype with a modal chromosome number of 84.
Notably, it demonstrates tumorigenic properties both in soft agar and nude mice, affirming its utility in cancer research, particularly in the context of prostate carcinoma.
Characterized by its adherent growth pattern, LNCaP clone FGC often forms single cells and loosely attached clusters rather than a uniform monolayer, necessitating mechanical dispersion during subculturing.
The cells exhibit a slow growth rate, light attachment to substrates, and a tendency to rapidly acidify the culture medium.
Initial post-subculture incubation is critical for optimal cell attachment and growth.
This cell line expresses key prostate cancer markers, including human prostatic acid phosphatase and prostate-specific antigen, alongside positive androgen and estrogen receptor expression.
Such markers underline its responsiveness to 5-alpha-dihydrotestosterone, which modulates growth and acid phosphatase production.
Given these characteristics, LNCaP clone FGC is an invaluable tool for advanced cancer research applications, including 3D cell culture and as a host for transfection studies, contributing to its high citation rate and utility in the field of human cell research.
