概要：The AsPC1 cell line was derived from a 62-year-old female patient with adenocarcinoma of the head of the pancreas and metastases to several abdominal organs.
Despite receiving radiation and chemotherapy, the patient developed ascites and passed away two weeks later.
The ascitic cell culture derived from this patient demonstrated abundant mucin and carcinoembryonic antigen 7 production.
In genetic analysis, KRAS was found to be activated.
In addition, ASPC-1 showed divergent results for SMAD4/DPC4.
The status of the tumor suppressor genes TP53 and CDKN2A/p16 was also inconsistent showing variable alterations in these genes.
It is suggested that these cells may have acquired additional alterations during routine culturing, and the heterogeneous populations in the original tumor could be a source of different genetic variants.
A study published in Neoplasia in 2016 investigated the efficacy of the HDAC inhibitor AR-42 in suppressing tumor growth in ASPC-1 models.
The results demonstrated tumor suppression and increased apoptosis, suggesting the potential of AR-42 as a treatment option.
Another study featured in Nature in 2016 examined the mechanism of action of the antimitotic and STAT3 inhibitor LTP-1 using ASPC-1 cells.
LTP-1 treatment induced cell cycle arrest, disrupted microtubule dynamics, and suppressed tumor growth, indicating its potential as a therapeutic agent.
In a 2006 study, ASPC-1 cells were used to characterize the mTOR inhibitor CCI-779 (temsirolimus) in human pancreatic cancer.
CCI-779 activated proteins involved in cell growth and exhibited antitumor effects in vivo.
Temsirolimus has since been FDA-approved for advanced kidney cancer treatment.
ASPC-1 cells have also played a crucial role in establishing carcinogenesis models for pancreatic ductal adenocarcinomas.
By manipulating oncogenes and establishing spheroid cultures of ASPC1 cells, researchers transformed normal human pancreatic duct cells into adenocarcinomas, providing valuable insights into the process of carcinogenesis.
ASPC-1 cells are a valuable resource for studying pancreatic ductal adenocarcinoma and possess specific markers associated with pancreatic cancer and have been utilized in various research studies exploring potential therapies.
