83-5599-49　SH-SY5Y Cells 1vial　300154

特徴

• 概要：SH-SY5Y cells, a subclone derived from the neuroblastoma cancer cell line SK-N-SH, established in 1970 from a biopsy of a metastatic bone tumor from a 4-year-old cancer patient, are a valuable cell model for neurodegenerative disorders such as Parkinsons and Alzheimers disease.
• These human cells, categorized as a homogeneous neuroblast, offer a unique cell source for functional studies in neurobiology and neurodegenerative disease research.
• The SH-SY5Y cell line, also referred to as the SY5Y cell line or SH cell line, is widely recognized for its utility in neuroscience, particularly as an in vitro model for Parkinsons disease research due to its adrenergic phenotype and the expression of dopaminergic markers, including dopamine.
• The neuronal differentiation of SH-SY5Y cells, which transform them into neuronal cell models resembling various functional neurons, is achieved through biochemical interconversion processes involving gradual serum deprivation, retinoic acid, neurotrophic factors like brain-derived neurotrophic factor, extracellular matrix proteins, and serial splitting.
• This differentiation is crucial for studying neuronal markers and conducting neurotoxicology and neurotoxicity assessments, especially concerning the impact of organic pollutants on human neuron-like cells.
• The cells response to neurotoxicology in humans offers significant insights into the safety data sheet download requirements for various compounds.
• SH-SY5Y cells growth mode is characterized by mixed growth properties, as they grow both adherently and in suspension, forming clusters during division that differ significantly from the morphology of differentiated cells.
• These undifferentiated cells, before undergoing neuronal differentiation, serve as an essential foundation for neuroscientific studies.
• The neurobiology of SH-SY5Y neuroblastoma cells is further enriched by the presence of choline acetyl transferase and markers for acetylcholinesterase and butyryl cholinesterase, which are pivotal in understanding cholinergic neurotransmission.
• The cytogenetics of these cells reveal a unique dna profile with a modal number of 47 chromosomes, including a distinctive marker of chromosome 1 characterized by a complex insertion resulting in trisomy of 1q.
• As a neurotoxicological model, the SH-SY5Y neuroblastoma cell line is instrumental in examining the effects of compounds on acetylcholinesterase and butyryl cholinesterase activities, essential for neurotoxicology studies.
• The sy5y lines contribution to understanding the biochemical pathways involved in neurodegenerative diseases, coupled with its role in the functional studies of dopaminergic and cholinergic systems, underscores its value in neuroscience research.
• However, researchers are advised to be cautious of the loss of neuronal characteristics with increasing passage numbers, recommending the use of cells before passage 20 or verifying specific characteristics such as noradrenaline uptake or neuronal tumor markers to ensure the integrity of their studies.