概要：The murine melanoma cell line B16-F10 is a subline of the B16 tumour line that was derived from the skin tissue of a C57BL/6J mouse.
These cells exhibit a spindle-shaped and epithelial-like morphology and are often used in skin cancer research.
Several sublines, for instance, B16-F1 and B16-F10, were derived from the mother B16 line by selection for their ability to form lung colonies in vivo after intravenous injection and subsequently established in vitro after one (B16-F1) or 10 (B16-F10) cycles of lung colony formation.
The cell size at rest is approximately 15.4 ?m with no statistical difference between B16-F1 and B16-F10 cells.
The doubling times of B16-F10 cells are approximately 20.1 hours.
B16-F10 possesses high lung metastatic ability, whereas B16-F1 is a subline with low metastatic potential.
B16-F10 melanoma cells have been widely used as a poorly immunogenic and highly aggressive model for murine tumor immunotherapy studies.
Invasive analysis shows that B16-4A5 is the most aggressive melanoma cell line for C57BL/6Js skin, followed by B16-F10 and a diminished aggressive growth pattern by the B16-GMCSF and B16-FLT3 cell lines.
Furthermore, melanin is released excessively by the tumor cells, and many metastases in lung and liver parenchyma were found in studies.
Regarding protein expression, inoculation of B16-F10 in mouse skin showed a perivascular predominance of VEGF expression on many vessels.
VEGF is a signal protein stimulating the growth of new blood vessels.
In B16-F10 inoculated test groups, S100, a protein associated with melanomas, was found heterogeneous with moderate to intense positivity.
