83-5599-06　［取扱停止］EL4 Cells 1vial　300653

特徴

• 概要：EL4 cells, derived from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene, are invaluable in biological science, particularly in immunology research.
• EL4 cells are also commonly employed in transfection experiments.
• In tumour biology, EL4 cells have proven instrumental in several studies.
• They have been utilized to induce tumours in mice, providing a valuable model system to investigate tumour development, progression, and therapeutic interventions.
• EL4 cells have also been used to assess the extravasation of tumour necrosis factor (TNF)-induced substances and evaluate the effectiveness of various therapeutic agents.
• EL4 cells grow in suspension, providing a convenient and scalable system for culturing large quantities of cells.
• With a doubling time of 12 to 20 hours, EL4 cells can rapidly expand, facilitating experimental studies that require ample cell numbers.
• EL4 cells have demonstrated their utility in assessing the tumoricidal activity of bone marrow-derived macrophages (BMDM).
• By incorporating EL4 cells into BMDM-polarizing media, researchers can evaluate the ability of macrophages to eliminate tumour cells.
• In terms of antigen expression, EL4 cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4), and Thy-1.2 antigen.
• EL4 cells exhibit a 100-fold greater expression of cell surface CD4 (CD4(high)) following retroviral transduction and G418 selection.
• The cells are resistant to cortisol at a concentration of 0.1 mM but sensitive to polyhydroxyalkanoates (PHAs) at 20 ?g/mL.