概要：CaD2 cells, originating from the murine breed C3H, represent a cancer cell line derived from a mouse mammary gland carcinoma.
Specifically, they were isolated from a six-month-old female mouse, closely mirroring the biological context of mammary gland malignancies in mice.
CaD2 cells offer a valuable platform for the study of malignant neoplasms due to their distinct attributes.
Their epithelial-like morphology and adherent growth properties ensures the reliability and meaningfulness of experimental results.
This robust characterization, combined with the availability of essential growth conditions, such as DMEM with 10% FBS, makes CaD2 a valuable resource in cancer research.
Researchers often maintain these cells at a recommended ratio of 1:3, with a seeding density of 5 x 10^4 cells/cm^2 to achieve a confluent monolayer within two days, allowing for regular experimentation, typically conducted 2 to 3 times per week.
The cells have been tested and confirmed to be negative for several viruses and microorganisms, including Sendai virus, Ektromelie virus, Polyoma virus, K-Virus, Kilham virus, Reo 3 virus, PVM (Pneumonia Virus of Mice), LCM (Lymphocytic Choriomeningitis) virus, M.pulmonis (Mycoplasma pulmonis), MVM (Minute Virus of Mice), Theilers GD VII virus, Toolans H-1 virus, MHV (Mouse Hepatitis Virus), LDV (Lactate Dehydrogenase-elevating Virus), RCV/SDA (Rat Coronavirus/Sialodacryoadenitis Virus), M-Adenovirus, and B.
piliformis.
This comprehensive testing ensures that CaD2 cells can be used with confidence in various research settings without concerns related to these potential contaminants.
