83-5598-83　L-929 Cells 1vial　400260

特徴

• 概要：L-929 cells are a type of adherent mouse fibroblast cell line often used as an alternate test system for toxicity assessment.
• NCTC clone 929 (L-929) is one of the first cloned strains developed from the parent L strain and was established from the 95th subculture generation using the capillary technique for single-cell isolation.
• The spontaneously immortalized parent L strain originates from the subcutaneous areolar and adipose tissue of a C3H/An mouse.
• L-929 cells are small in size, ranging from 5-10 micrometres, and exhibit adherent growth properties.
• The karyotype of these cells includes a modal chromosome number of 66, with a range of 65 to 68, and approximately 20 to 30 marker chromosomes present in each metaphase spread.
• However, cytogenetic instability has been reported in the literature for some cell lines.
• These cells have been shown to be tumorigenic in immunosuppressed mice and susceptible to viruses such as vesicular stomatitis and encephalomyocarditis.
• Additionally, L-929 conditioned medium has been found to be an alternative source of macrophage colony-stimulating factor (CSF-1), a cytokine that stimulates the growth, differentiation, and survival of monocytes, macrophages, and their progenitor cells.
• In a study on the photosensitivity of L-929 cells towards ultraviolet radiation (UVR), these cells were exposed to various intensities of UVA, UVB, and sunlight.
• The results showed that L-929 cells are photosensitive towards UVR.
• Analysis of the protein composition of the L929 supernatant identified 2,193 proteins with different expression rates, and the top 100 most abundant proteins make up more than 60% of the total protein content.
• The highly expressed protein was M-CSF, while other cytokines, such as macrophage MIF, were also identified alongside chemo-attractants and chemokines.
• However, neither IFN-gamma nor IFN-? was detectable during the supernatant collection period, disputing previous studies that reported the production of type 1 interferons by L929 fibroblasts.
• In conclusion, L-929 cells are a valuable tool in biological science for toxicity assessment and as an alternative source of macrophage colony-stimulating factors.
• The protein composition of the L929 supernatant provides valuable insight into the potential activation state during the differentiation of BMDMs.