83-5598-81　E11 Cells 1vial　400494

特徴

• 概要：The E11 cell line has been meticulously cloned from the outgrowth of glomeruli, which were isolated from H-2kb-tsA58 transgenic mice.
• These transgenic mice carry a temperature-sensitive variant of the SV40 large T antigen, under the precise control of the IFN-g-inducible H-2kb promoter.
• This intricate genetic arrangement ensures the controlled development and sustained viability of the E11 cell line.
• One of the most remarkable attributes of the E11 cell line is its exceptional stability.
• Researchers have successfully propagated these cells for more than 40 passages without any observed phenotypic alterations.
• This enduring stability has facilitated rigorous experimentation and in-depth research, making the E11 cell line an ideal candidate for studies in podocyte biology.
• These cells exhibit stable expression of nephrin, a pivotal protein closely associated with podocytes.
• Furthermore, they display a remarkable capacity for forming abundant cell-cell contacts, a critical characteristic for mimicking the behavior of podocytes in their native environment.
• E11 cells not only stably express nephrin but also demonstrate the presence of a spectrum of other essential podocyte proteins.
• This comprehensive protein expression profile includes NEHP1, FAT, P-cadherin, podocin, CD2AP, ZO-1 (?-isoform), Lmx1b, podoplanin, synaptopodin, cortactin, and vimentin.