83-5598-54　［取扱停止］GC-1 spg Cells 1vial　300375

特徴

• 概要：The immortalized cell line GC-1 spg originates from the testis of a transgenic mouse engineered with the SV40 early region.
• Distinct cell types contributing to the seminiferous tubule in the mouse testis have been immortalized and cultivated, resulting in a diverse collection of cell lines.
• This includes 16 peritubular cells, 22 Leydig cells, 8 Sertoli cells, and one germ cell line, GC-1 spg, all of which have shown exceptional growth and consistency throughout the culture period.
• Developed through the immortalization process facilitated by the SV40 large T antigen, GC-1 spg cells have demonstrated exceptional stability and viability.
• For 2.5 years, these cells have been cultured for an impressive 90 generations, showcasing their remarkable longevity and suitability for long-term studies.
• The establishment of the GC-1 spg germ cell line represents a significant achievement.
• These cells occupy a crucial developmental stage, bridging the gap between spermatogonia type B and primary spermatocytes.
• Their distinct morphological and molecular features, observed through phase contrast and electron microscopy, allow for precise identification.
• Additionally, GC-1 spg cells express the testicular cytochrome ct and lactate dehydrogenase-C4 isozyme, confirming their resemblance to in vivo counterparts.
• Given their tissue origin and their striking similarity to spermatocytes, GC-1 spg cells hold immense potential for various applications, with a particular focus investigations in reproductive biology.
• Researchers can utilize these cells to study the intricate processes involved in male germ cell development, uncovering valuable insights into reproductive biology and fertility.