83-5598-49　C2C12 Cells 1vial　400476

特徴

• 概要：C2C12 cells are an immortalized mouse myoblast cell line commonly used in biomedical research.
• These cells were initially obtained from the thigh muscle of a 2-month-old regular mouse and were developed for in vitro studies of myoblasts to isolate complex interactions of in vivo conditions.
• C2C12 cells can rapidly proliferate under high serum conditions and can differentiate into myotubes, the precursors of contractile skeletal muscle cells, under low serum conditions or starvation.
• One of the significant advantages of C2C12 cells is their ability to incorporate exogenous cDNA and nucleic acids through transfection.
• This has made these cells a popular choice in gene expression studies and enabled researchers to study the differentiation of myoblasts, osteoblasts, and myogenesis and explore various biochemical pathways.
• C2C12 cells can undergo rapid development and maturation into functional skeletal muscle cells.
• These cells can differentiate by replacing growth media with differentiation media, forming fused cells within 24 hours.
• Myogenic markers such as Myf5, MyoD, Myogenin, Mrf4, and muscle-specific markers such as Csrp3, Hes6, Mef2a, and Mef2d are indicative of C2C12 cell differentiation.
• The gene C6ORF32 is upregulated during the differentiation process and may play a role in myogenic cells migration toward the fusion site.
• The wild-type C2C12 cells have a radial branching morphology consisting of long fibers extending in many directions.
• The growth patterns of these cells can be altered by introducing adhesion molecules and other factors that can affect the cytoskeleton and shape of the cells.
• The scaffolding of C2C12 cells is significant for studying muscle tissue regeneration after injury or in cases of tissue wasting due to disease or intensive care unit rehabilitation.
• In conclusion, C2C12 cells are an essential tool for biomedical research and have been used to study a wide range of biological processes, including muscle formation, differentiation, and gene expression.