概要：Originally derived from BALB/c embryonic liver cells, BNL CL.2, a mouse liver cell line, has been utilized for the characterization of cyclin-dependent kinase (CDK) protein complexes.
Moreover, researchers have leveraged this cell line to study the changes in CDK protein complexes following both chemical and viral transformation.
This cell line has a broader relevance as it serves as the progenitor for various transformed cell lines, including 3-Methylcholanthrene epoxide-transformed (BNL 1ME A.7R.1), MNNG-transformed (BNL 1NG A.2), and SV40-transformed (BNL SV A.8) liver cells, all of which stem from BNL CL.2.
These transformed cell lines have proven to be valuable for assessing the alterations in CDK protein complexes subsequent to transformations induced by chemical and viral agents.
Importantly, these cell lines have been thoroughly characterized, initially by Patek et al., providing a robust foundation for research in the field.
The distinguishing feature of BNL CL.2 lies in its non-tumorigenic nature in immunosuppressed mice and the inability to exhibit anchorage-independent growth.
However, BNL CL.2 cells do possess the capability to form colonies when cultured in semisolid medium, making them a valuable model for studying certain cellular processes and transformations.
In contrast, MC-, MNNG-, and SV40-transformed liver cells, derived from BNL CL.2, exhibit the capacity to grow in soft agar and form tumors in immunodeficient mice.
The specific characteristics of BNL CL.2, its non-tumorigenic properties, and its ability to form colonies in semisolid medium make it a valuable resource in the realm of stable cell transfection and cellular biology research.
