83-5597-96 NCI-H82 Cells 1vial 300442
特徴
概要：The NCI-H82 cell line was derived by A.F.
Gazdar and associates in 1978 from the pleural fluid of a patient with small cell cancer of the lung.
The morphology of the original tumor was not characteristic of SCLC.
The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase.
It does not have detectable levels of L-DOPA decarboxylase or bombesin.
The cells produce an abnormally sized p53 mRNA (3.7 kb).
C-myc DNA sequences are amplified about 25 fold, and there is a 24 fold increase in c-myc RNA relative to normal cells.
The cells are reported to express functional ANP receptors, but treatment with ANP does not alter their growth pattern.
The cells stain positively for neurofilaments and vimentin.
There is expression of v-fes, v-fms, Ha-ras, Ki-ras, N-ras and c-raf 1 mRNAs.
仕様
容量：1vial
カテゴリー：Lung cancer cell lines
Organism：Human
Tissue：Lung
Disease：Lung small cell carcinoma
Metastatic Site：Pleural effusion
Synonyms：NCI-H-82, H82, H-82, NCI H82, NCIH82, H82sclc
Age：41 years
Gender：Male
Ethnicity：Caucasian
Morphology：Epithelial-like
Growth Properties：Aggregates in suspension. The cells grow in very large aggregates, which are the only viable cell population in the culture.
Citation：NCI-H82 (Cytion catalog number 300442)
Biosafety Level：1
Receptors Expressed：Insulin-like growth factor II receptor (IGF II), atrial natriuretic peptide (ANP)
Protein Expression：p53 positive
Isoenzymes：G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, Me-2, 1, AK-1, 1, GLO-1, 1, Phenotype Frequency Product = 0.0082
Tumorigenic：Yes, forms transplantable tumors with non-typical SCLC histology in nude mice
Karyotype：This is a near triploid human cell line. The modal chromosome number is 58, occurring at 44% with polyploidy at 3%. Each cell had two copies of a normal x chromosome. The Y chromosome was not detected in Q banded preparations.
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