Gazdar and associates from the pleural fluid of a patient with small cell cancer of the lung.
The original tumor morphology was not characteristic of small cell lung cancer.
The cell line is a variant small cell lung cancer in biochemistry and morphology, and expresses neuron specific enolase as well as the brain isoenzyme of creatine kinase.
None of L-DOPA decarboxylase, bombesin, vasopressin, oxytocin or gastrin releasing peptide has been detected in the cell line.
This cell line exhibits a 20-fold higher degree of c-myc DNA amplification and a 15-fold higher degree of c-myc RNA.
The cell line was originally propagated in serum free RPMI 1640 medium supplemented with 10 nM of hydrocortisone, 5 microgram/mL of insulin, 10 microgram/mL of transferrin, 10 nM of 17-beta-estradiol, and 30 nM of sodium selenite.
Transplantable tumors with non-typical small cell lung cancer histology can be formed by the cells.
仕様
容量：1vial
カテゴリー：Lung cancer cell lines
Organism：Human
Tissue：Lung
Disease：Lung small cell carcinoma
Metastatic Site：Pleural Effusion
Synonyms：NCI-H446, H-446, NCI-446, NCIH446
Age：61 years
Gender：Male
Ethnicity：European
Morphology：Epithelial-Like
Growth Properties：Adherent/suspension
Citation：H446 (Cytion catalog number 305049)
Biosafety Level：1
Tumorigenic：Yes, in nude mice (The cells form transplantable tumors with non-typical SCLC histology).
