概要：The NCI-H441 cell line was established in 1982 from pericardial fluid associated with papillary adenocarcinoma of the lung in a male patient.
Characterized by an epithelial morphology, these cells adhere to surfaces and exhibit a hyperdiploid karyotype with a modal chromosome number of 52, although variations ranging from 44 to 59 have been observed.
This variation includes a high frequency of cells with 53 chromosomes and a 4.9% occurrence of cells with even higher ploidies.
Notably, over 14 marker chromosomes are consistently present across all cells, with an additional six or more observed in certain cells, including specific translocations and deletions such as der(1)t(1|1)(p36|p21) and i(2q), among others.
NCI-H441 cells are utilized extensively in biological research due to their relevance to lung tissue and disease, particularly adenocarcinoma.
They serve as a crucial model for 3D cell culture, high-throughput screening, and toxicology studies, offering insights into cellular responses and drug efficacy.
A specific application includes their use as a transfection host for the expression of pulmonary surfactant protein (SP-B), underlining their importance in respiratory research.
The cell lines genome and isoenzyme profile, with markers such as AK-1, ES-D, G6PD B, and others, supports its unique identity and utility in research.
Electron microscopy reveals the presence of multilamellar bodies and structures akin to clara cell granules, indicative of their lung epithelial origin.
Additionally, these cells are capable of cloning in soft agar, with or without serum, demonstrating their adaptability and resilience in various experimental conditions.
The expression of mRNA and protein for major surfactant apoprotein (SP-A) further emphasizes their role in studying lung function and diseases, particularly those affecting surfactant regulation and synthesis.
