83-5597-39　HuH7 Cells 1vial　300156

特徴

• 概要：HuH-7 cells are a type of epithelial-like, tumorigenic cell line initially taken from a liver tumor in a 57-year-old Japanese male in 1982.
• The human hepatoma-derived HuH-7 cell line and its derivatives have been widely used in research as a convenient experimental substitute for primary hepatocytes.
• In particular, they have been instrumental in hepatitis C research and used as host cells for propagating the virus in vitro.
• HuH-7 cells have played a crucial role in hepatitis C research, especially when it comes to drug development.
• Prior to 2005, researchers were unable to cultivate the hepatitis C virus in the laboratory, making it difficult to test potential drug candidates against it.
• The introduction of the HuH-7 cell line changed that.
• These cells are highly permissive to the replication of the hepatitis C virus, making them ideal for in vitro testing.
• By using the HuH-7 cells, researchers were able to screen drug candidates against laboratory-grown hepatitis C, which paved the way for the development of new drugs to fight the virus.
• Unlike other established human hepatoma cell lines, HuH-7 cells can be propagated in a chemically defined medium containing trace amounts of selenium in place of serum.
• This allows for systematic studies of the in vitro effects of various compounds on their growth and metabolism.
• Most HuH-7 cells have a chromosome number between 55 and 63 and typically grow as 2D monolayers.
• The growth medium for HuH-7 cells should be renewed 2-3 times a week or as needed according to the media pH, and cell confluency should be maintained between 30 to 90%.
• The doubling time of these cells is 24 to 50 hours.
• In comparison to HepG2 cells, which have been commonly used as a model for the study of synthesis and secretion of human apoB-100, Huh-7 cells were adopted as an alternative model with the assumption that they would be superior in some respects of lipoprotein metabolism, including VLDL secretion.
• However, it was found that Huh-7 cells did not offer any advantages over HepG2 cells as a general model of human apoB100-lipoprotein metabolism.