特徴
- 概要：HepG2 cells, a hepatoblastoma cell line, are a cornerstone in biological science, particularly in liver cancer research.
- The HepG2 cell line was first isolated in 1975 and initially misclassified as hepatocellular carcinoma, with the HepG2 cell line origin as hepatoblastoma being recognized later, clarifying years of scientific ambiguity.
- Human hepatic cell lines such as HepG2 are commonly used as in vitro models for primary human hepatocytes.
- These cell lines offer advantages such as indefinite proliferation, stable phenotype, easy accessibility, and ease of manipulation.
- However, they exhibit reduced expression of some metabolic functions compared to primary hepatocytes.
- Derived from hepatocellular carcinoma, HepG2 cells proliferate quickly and have an epithelial-like morphology, performing many specialized hepatic functions.
- Despite these differences, HepG2 cells are widely used in studying drug metabolism and toxicity, thanks to their resemblance to hepatocellular carcinoma and hepatoblastoma cells in terms of drug metabolism and transport proteins.
- HepG2 is a human liver cancer cell line often used in research, including studies on drug metabolism and toxicity.
- However, one of the limitations of hepatoma HepG2 cells is their altered expression of certain liver-specific functions, including the expression of cytochrome P450 enzymes.
- Cytochrome P450 enzymes are essential for the metabolism of xenobiotics (foreign compounds such as drugs and carcinogens) in the liver.
- The altered or reduced expression of these enzymes in HepG2 cells can affect their ability to accurately model the metabolism and elimination of xenobiotics, which is a critical aspect of liver function.
- The HepG2 cell line, alongside other hepatoma cell lines such as the Hep3B and human hepatoma HepaRG cell lines, contributes to a broader understanding of human liver carcinoma cells.
- The cell line stands out for its versatility, serving as an optimal choice for stable cell line generation, transfection studies, drug metabolism, and hepatotoxicity studies.
- Furthermore, the HepG2 cell line is pivotal in a range of applications, from 3D cell culture to high-throughput screening and toxicology.
仕様
- 容量：1vial
- カテゴリー：Liver cancer cell lines
- Organism：Human
- Tissue：Liver
- Disease：Hepatocellular carcinoma
- Applications：This cell line is an optimal choice for transfection. Further, the HepG2 cells offer an array of applications, ranging from 3D cell culture and cancer research to high-throughput screening and toxicology.
- Synonyms：HEP-G2, Hep G2, HEP G2, Hep-G2, HEPG2
- Age：15 years
- Gender：Male
- Ethnicity：Caucasian
- Morphology：Epithelial-like
- Growth Properties：Adherent
- Citation：HepG2 (Cytion catalog number 300198)
- Biosafety Level：1
- Receptors Expressed：insulin, insulin-like growth factor II (IGF II)
- Protein Expression：p53 positive
- Tumorigenic：no
- Products：Albumin, alpha-fetoprotein (alpha fetoprotein), alpha1 acid glycoprotein (alpha-1 acid glycoprotein), alpha1 antitrypsin (alpha-1-antitrypsin), alpha1 antichymotrypsin, (alpha-1-antichymotrypsin), alpha2 HS glycoprotein (alpha-2-HS- glycoprotein), alpha2 macroglobulin (alpha-2-macroglobulin), beta lipoprotein (beta-lipoprotein), ceruloplasmin, C4 and C3 activator, fibrinogen, haptoglobin, plasminogen, retinol binding protein (retinolbinding protein), transferrin
- Karyotype：modal number = 55 (range = 50 to 60), has a rearranged chromosome 1
- 非営利団体（アカデミア）様向けの価格です。営利企業様や営利目的での販売は実施しておりません。
- 購入にはメーカーの「Non Profit Supply Agreement」及び弊社の「ヒト生体試料の提供に関する同意書」への記入が必要となる場合がございます。
- この商品は法規制を確認しておりません｡(法規制によって販売できない場合もございます)
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
よくあるご質問（FAQ）
掲載カタログ情報
| 掲載カタログ名 | 掲載ページ |
|---|