概要：Hep3B is a human hepatoma cell line isolated from liver tissue derived from an 8-year-old child with liver cancer.
These cells exhibit epithelial morphology and contain an integrated hepatitis B virus genome.
Hep3B cells are commonly used in drug metabolism and hepatotoxicity studies.
In contrast to HepG2 cells, Hep3B cells are hepatitis B virus-positive and tumorigenic.
Hep3B cells have an average of 60 chromosomes per cell, while HepG2 cells contain an average of 55 (50?56) chromosomes per cell.
Moreover, HepG2 cells have a rearranged chromosome 1, while Hep3B cells do not.
In terms of gene expression, the Hep3B cell line expresses alpha-fetoprotein (AFP), hepatitis B surface antigen (HBsAg), albumin, alpha-2-macroglobulin, alpha-1-antitrypsin, transferrin, alpha-1-antichymotrypsin, haptoglobin, ceruloplasmin, plasminogen complement (C3, C4), C3 activator, fibrinogen, alpha-1 acid glycoprotein, alpha-2-HS-glycoprotein, beta-lipoprotein, and retinol-binding protein.
However, the Hepatitis B Viral Protein HBx, which regulates the expression of the majority of genes differentially expressed between HepG2 and Hep3B, may not be the crucial factor in determining the differences between these cell lines.
Instead, the different cancer maturation stages are considered to be responsible for the differences between HepG2 and Hep3B.
仕様
容量：1vial
カテゴリー：Liver cancer cell lines
Organism：Human
Tissue：Liver
Disease：Childhood hepatocellular carcinoma
Synonyms：Hep 3B2_1-7, HEP3B217, Hep 3B2, HEP-3B2, HEP3B2, Hep-3B, HEP-3B, Hep 3B, Hep3B, HEP3B
Age：8 years
Gender：Male
Ethnicity：African
Morphology：Epithelial
Growth Properties：Adherent
Citation：Hep 3B2.1-7 (Cytion catalog number 305141)
Biosafety Level：1
Protein Expression：Alpha Fetoprotein(Alpha-Fetoprotein), Hepatitis B Surface Antigen(Hbsag), Albumin, Alpha2 Macroglobulin(Alpha-2-Macroglobulin), Alpha1 Antitrypsin(Alpha-1-Antitrypsin), Transferrin,, Alpha1 Antichymotrypsin(Alpha-1-Antichymotrypsin), Haptoglobin, Cerulopl
