83-5597-29　［取扱停止］HEK293 suspension-adapted 1vial　300686

特徴

• 概要：The adaptation of the HEK293 cell line to suspension culture resulted in robust growth and achieved similar or improved maximum cell density compared to adherent cultures.
• This transition to suspension culture simplifies subcultivation, enhances scalability, and reduces production costs, making it an advantageous approach for large-scale manufacturing of biotechnological products.
• The HEK293 cell line, initially derived in the 1970s, arose from the transformation of human embryonic kidney cells with sheared adenovirus 5 DNA, leading to approximately 30% of cells exhibiting hypotriploid karyotypes with 64 modal chromosomes and 4.2% with higher ploidies.
• The multiple copies of adenovirus E1A and E1B genes in HEK293 cells result in high transfection efficiency, making them valuable in biotechnology for protein expression, gene therapy, and vaccine production.
• Moreover, the pharmaceutical industry employs HEK293 cells for high-throughput screening assays due to their ability to be transfected with plasmids encoding drug targets and cultured in large numbers.
• In industrial biotechnology, HEK293 cells serve as hosts for producing recombinant proteins, enzymes, and biosensors.
• Additionally, HEK293 cells are essential in toxicology research for studying the effects of chemicals and environmental toxins on cellular processes and gene expression.
• Notably, the HEK293 cell line is also utilized to generate recombinant viruses like adenovirus and lentivirus for gene therapy, viral replication, and host-virus interactions.
• Overall, the suspension-adapted HEK293 cell line, along with its versatile applications in biomedical research, biotechnology, pharmaceuticals, and toxicology, signifies a significant advancement in the field of cell culture technology.