概要：HCT116 cells, isolated from an adult male colon cancer patient, serve a crucial role in therapeutic studies and drug screenings, particularly in colon cancer research.
HCT-116 cells are recognized for a mutation in codon 13 of the KRAS proto-oncogene, highlighting their utility in gene therapy research, especially since they are amenable to transfection with viral vectors.
Epithelial by nature, HCT 116 cells demonstrate the ability to metastasize in xenograft models, which is pivotal for tumorigenicity studies.
Their karyotype is nearly diploid, with 70% of the cells harboring 45 chromosomes, often showing an overrepresentation of chromosomes 8, 10, 16, and 17 on the long arms, along with the absence of the Y-chromosome.
The application of the colorectal carcinoma cell line HCT 116 extends to various biomedical investigations, including the study of Cyclin D1s role in lithocholic acid hydroxyamide activity and the impact of differing transferrin levels within polyethyleneimine-siRNA complexes on siRNA uptake.
These cells are instrumental in examining the selective uptake of Quilamine HQ1-44, an iron chelator, via the polyamine transport system.
Notably, the HCT116 cell line exists in two variants, one with high Insp8 gene expression and one without, affecting cellular metabolism and phenotype.
In apoptosis research, HCT116 cells are pivotal for apoptosis analysis, studying cellular apoptosis, and understanding the mechanisms of apoptosis and cell death.
The effects of butyrate, a short-chain fatty acid, have been extensively studied in HCT116 cells, revealing that butyrate inhibits colon cancer proliferation by inducing apoptosis, highlighting the intricate cancer cell interaction and the broader implications for cancer research.
Butyrates role in modulating gene expression changes and inducing endoplasmic reticulum stress response in HCT116 cells underscores the cellular complexity in colorectal cancer cell lines.
This research contributes to the cell line catalogue and cell line ontology, enriching the knowledge base of human cell lines used in cancer research.
The interaction between HCT116 colon cancer cells and therapeutic agents like metformin, known for its legacy effect and potential to reduce the risk of cancer, is of significant interest.
Metformins influence on HCT116 colon cell proliferation, p21 protein level modulation, and its broader implications on proliferation and growth offer insights into the management of primary tumors and the prevention of tumors and metastases.
Furthermore, the study of HCT116 cells in the context of colorectal cancer, including the exploration of colorectal carcinoma and colorectal carcinoma cell line dynamics, enriches the understanding of cancer biology.
The use of high glucose conditions, EDTA solution in experimental setups, and the development of stable reporter cell lines, such as IRF reporter cells, further diversify the experimental approaches in studying HCT116 cells.
Quality control specifications for these cell lines ensure the reliability of data, particularly in studies focused on colorectal cancer cells, colorectal cancer proliferation, and the broader spectrum of human HCT116 research.
The integration of these keywords into the study of HCT116 cells not only expands the scope of research but also enhances the understanding of colorectal cancer and its treatment.
Antigen Expression：The cells are positive for keratin by immunoperoxidase staining. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression.
Tumorigenic：Yes, in nude mice (inoculum of 5-10 x 106 cells)
Ploidy Status：Aneuploid
Msi Status：Instable (MSI-high)
Karyotype：The karyotype of HCT116 cells is nearly diploid, with 70% of the cells harboring 45 chromosomes, often showing an overrepresentation of chromosomes 8, 10, 16, and 17 on the long arms, along with the absence of the Y-chromosome.
