概要：The colorectal carcinoma cell line LS-513 was isolated in 1985 from a primary tumor biopsy of a 63 year old Caucasian male patient.
He was diagnosed with a Dukes C mucin secreting cecal tumor located at the Bauhin valve.
LS-513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin.
MHC class II antigens (HLA-DR, DQ, and DP were not detected).
TGF beta-1 is inhibitory for proliferation of LS-513 cells, whereas TGF beta-2 has no effect on the growth of these cells.
LS-513 cells are 100-fold less sensitive to TGF beta-1 than the LS-1034 cell line.
LS-513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice.
Colony forming efficiency was 30% in methylcellulose.
仕様
容量：1vial
カテゴリー：Intestine cancer cell lines
Organism：Human
Tissue：Colorectal
Disease：Adenocarcinoma
Synonyms：LS513, LS 513
Age：63 years
Gender：Male
Ethnicity：Caucasian
Morphology：Epithelial-like
Growth Properties：Adherent
Citation：LS-513 (Cytion catalog number 300457)
Biosafety Level：1
Protein Expression：CEA+ (50%), p53+
Antigen Expression：Carcinoembryonic antigen (CEA), ICAM-1, HLA class I positive
Tumorigenic：Yes, forms tumors in nude mice
Products：Transforming growth factor beta 1 (TGF beta-1, 83 pg per 10 exp6 cells per 24 hours)
Karyotype：Two stem lines can be distinguished. The main one was represented in 65% of the cells, with a modal number of 51,xY and 3 markers, M1 - der(1)t(1,15), M2 - der(2)t(2,3)der(3)t(2,3), M3, and a monosomy 15. The second stem line had a modal chromosome number of 52,xY and presented M2 and M3 plus an isochromosome for the long arm of chromosome 1 called M4. A trisomy 5,7, a tetrasomy 13, and a monosomy 2 and 3 were present in all of the cells analyzed, the line did not exhibit monosomy 15.
