特徴
- 概要：HT-29 cells are a well-known human colorectal adenocarcinoma cell line widely used in biological and medical research.
- These cells were first isolated from a primary colorectal tumor in a 44-year-old Caucasian female in 1964 and have since become a valuable resource for researchers looking to study the biology of human cancers.
- One of the key features of HT-29 cells is their sensitivity to standard chemotherapy drugs for colorectal cancer, such as 5-fluorouracil and oxaliplatin.
- Additionally, these cells express characteristics of mature intestinal cells, such as enterocytes and mucus-producing cells, which has led to their use in studies focused on food digestion and bioavailability.
- Under standard growth conditions, HT-29 cells display an undifferentiated phenotype and consume high amounts of glucose.
- However, when grown in a glucose-free medium or treated with different inducers like butyrate or acid, these cells can be modulated to express different pathways of enterocyte differentiation, making them a valuable model for studying the molecular mechanisms involved in cell differentiation.
- Morphological, physiological, and biochemical markers, including the formation of a monolayer of cells with tight junctions and a typical apical brush border, characterize the differentiated phenotype of HT-29 cells.
- These differentiated cells express brush-border-associated hydrolases distinct from the small intestine, have brush-border microvilli, and can produce a relatively high level of mucin.
- Stepwise adaptation of exponentially growing HT-29 cells to increasing concentrations of methotrexate (MTx) results in the transformation of these cells into mucus-secreting differentiated cells, which can be used in the transport studies of different compounds, the study of the mucus-inducing properties of varying food compounds, or in studies regarding microorganism adhesion and survival.
- The differentiated HT-29 cells exposed to methotrexate are named HT29-MTx and have been characterized concerning tight junction formation, development of confluent monolayers, and production of a mucous layer.
- Overall, HT-29 cells have proven to be a valuable tool for researchers looking to study the biology of human cancers and the molecular mechanisms involved in cell differentiation.
- They continue to be widely used in medical and biological research to this day.
仕様
- 容量：1vial
- カテゴリー：Intestine cancer cell lines
- Organism：Human
- Tissue：Colon
- Disease：Adenocarcinoma
- Synonyms：HT 29, HT29
- Age：44 years
- Gender：Female
- Ethnicity：Caucasian
- Morphology：Epithelial-like
- Growth Properties：Adherent
- Citation：HT-29 (Cytion catalog number 300215)
- Biosafety Level：1
- Receptors Expressed：urokinase receptor(u-PAR), vitamin D (moderate expression), no detectable plasminogen activator activity.
- Protein Expression：CEA negative, p53 positive
- Antigen Expression：Blood Type A, Rh+, HLA A1, A3, B12, B17, Cw5, CD4 -, cell surface expression of galactose ceramide (a possible alternative receptor for HIV)
- Isoenzymes：Me-2, 1, PGM3, 1-2, PGM1, 1-2, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0230
- Oncogenes：myc+, ras+, myb+, fos+, sis+, p53+, abl -, ros -, src ?
- Tumorigenic：Yes, in nude mice. Forms well differentiated adenocarcinoma consistent with colonic primary (grade I), tumors also form in steroid treated hamsters
- Virus Susceptibility：human immunodeficiency virus (HIV, LAV)
- Products：Secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein, mucin, The p53 antigen is overproduced
- Karyotype：The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-,?) with a deleted short arm), t(7q,?), t(10q,?), i(13q), 19q+a. M6, ?t(8q,9q-), ?xp, M9, 6q+, t(13,?)a, t(13,?)b, 19q+b, M14, M15, 15p+, and xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
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