83-5596-03　［取扱停止］HUVEC, single donor 1vial　300605

特徴

• 概要：HUVECs, or human umbilical vein endothelial cells, are primary cells commonly used in biomedical research to study vascular biology in vitro.
• These cells are isolated from the umbilical cord vein and are a model system for studying endothelial cell function.
• Endothelial cells line the inside of blood vessels and play a critical role in vascular homeostasis, angiogenesis, and various vascular diseases such as atherosclerosis.
• One of the key advantages of using HUVECs in research is their ability to closely mimic the vasculature and study endothelial cell dynamics in vitro.
• This makes them an important tool in various research fields, including inflammation, oxidative stress, infection response, and normal and tumor-associated angiogenesis.
• In cancer research, HUVECs are of particular interest due to the ability of tumors to induce angiogenesis.
• Solid tumors require a healthy supply of oxygen- and nutrient-rich blood to grow and survive, and angiogenic processes direct the formation of new blood vessels, enhancing the tumor blood supply.
• Developing therapeutic agents that target tumor angiogenesis is an active area of research, and HUVECs play a significant role in this process.
• HUVECs are a primary, non-immortalized cell system that is easy to isolate and culture, making them an ideal model for studying vascular endothelium.
• They can be used in standardized in vitro angiogenesis assays to study normal endothelial cell behavior or evaluate how endothelial cells react to different stimuli or treatments with pro- and anti-angiogenic agents.
• HUVECs can also be used in tube formation assays to assess the ability of endothelial cells to form tubes.
• This process occurs after endothelial proliferation and migration to the site of the pro-angiogenic stimulus.
• To culture HUVECs, it takes about 2-4 hours to attach to the surface with 0.2% gelatin coating.
• Our HUVEC cells have undergone extensive testing to ensure their quality and viability.
• We guarantee that each vial contains at least 70% viable cells that have been tested for the presence of mycoplasma, bacteria, yeast, or other fungi.
• Quality control includes testing for the absence of hepatitis B, hepatitis C, and HIV-1 viruses.
• Our HUVEC cells are cryopreserved at the end of the primary culture phase in a medium containing 10% DMSO, with each vial containing over 0.5 x 10^6 viable cells.
• In conclusion, HUVECs are a widely used model system to study vascular biology in vitro.