概要：BJ cells, derived from neonatal male foreskin, are human fibroblasts with a morphology characteristic of their cell type.
Originating from normal skin tissue, these cells hold significant value in biological research due to their human origin and fibroblast properties.
They are particularly utilized in fields such as 3D cell culture, high-throughput screening, and toxicology, highlighting their versatility in experimental applications.
A key feature of BJ cells is their adherence to growth surfaces, a trait essential for numerous in vitro studies, including those involving stable transfection.
This capability allows for the exploration of gene function and expression in a controlled environment, providing insights into cellular responses and interactions.
Notably, BJ cells maintain a normal diploid karyotype up to population doubling 61, ensuring genetic stability within this range.
However, beyond population doubling 82, an abnormal karyotype is observed, indicating genetic alterations that may influence experimental outcomes.
The absence of telomerase activity in these cells further underscores their utility in studying cellular aging and senescence mechanisms, reflecting their relevance in aging-related research and toxicological assessments.
