概要：hFOB 1.19 is a unique osteoblast cell line derived from fetal limb tissue.
It was established through transfection with the temperature-sensitive expression vector pUCSVtsA58 and the neomycin resistance expression vector pSV2-neo.
Clones were selected in the presence of 0.6 mg/mL G418, ensuring genetic stability.
hFOB 1.19 are characterized by its stable genetic makeup, thanks to the use of pUCSVtsA58 and pSV2-neo during transfection.
As this cell line is derived from fetal tissue, it may provide insights into early osteoblast development.
hFOB 1.19 cells were immortalized through SV40 large T antigen transformation, allowing for extended culture and experimentation.
With a diploid karyotype in 43% of cases and a tetraploid karyotype in 57%, these cells offer variability for research applications.
hFOB 1.19 cells express alkaline phosphatase, a key marker for osteoblast differentiation and function.
At 33.5 degree, these cells exhibit rapid division (36-hour doubling time).
At 39.5 degree, division slows (96-hour doubling time), promoting differentiation and a more mature osteoblast phenotype.
hFOB 1.19 cells can differentiate into mature osteoblasts, expressing the typical osteoblast phenotype.
Cultured at higher temperatures, they shift toward differentiation, making them ideal for studying osteoblast function.
