概要：Breast cancer is one of the leading causes of cancer-related deaths among women worldwide.
Various cell models have been developed to understand better breast cancer tumorigenesis, metastasis, and drug sensitivity.
Among these models, the MCF10A human mammary epithelial cell line is a widely used in vitro model for studying normal breast cell function and transformation.
The MCF10A cell line was isolated in 1984 from the mammary gland of a White, 36-year-old female with fibrocystic breasts.
The Michigan Cancer Foundation deposited this cell line.
The MCF10A human breast epithelial cell line is arguably the most commonly used normal breast cell model.
These cells were derived from the benign proliferative breast tissue and spontaneously immortalized without defined factors.
The MCF 10A cell line is a non-tumorigenic epithelial cell line.
They exhibit three-dimensional growth in collagen and form domes in confluent cultures.
The cells are positive for epithelial sialomucins, cytokeratins, and milk fat globule antigens.
They also express breast-specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).
By electron microscopy, the cells display luminal ductal characteristics but not myoepithelial cells.
The calcium content of the medium exerts a substantial effect on the morphology of the cells.
The MCF10A cells have shown no signs of terminal differentiation or senescence.
MCF10A cells are widely used non-malignant breast epithelial cells.
They exhibit some features of normal breast epithelium, including a lack of anchorage-independent growth and dependence on growth factors and hormones for proliferation and survival.
They can form acinar structures in 3D culture, which makes them an excellent model to analyze the effects of oncogenes.
MCF10A cells exhibit a basal-like phenotype but express luminal and stem-like markers in 2D culture and present a unique epithelial-cell marker expression profile.
仕様
容量：1vial
カテゴリー： Human cells | Spontaneously immortalized cell lines
