概要：The epithelial cell line HBL-100 has been derived by E.V.
Gaffney and associates from the milk of a nursing mother and obtained 3 days after delivery.
Although there was no evidence of a breast lesion in the milk donor, and the patient had no family history of breast cancer, the karyotype of the recovered cells was abnormal as early as passage 7.
This line was able to synthesize a small amount of lactose and would respond to prolactin or estrogen by producing increased amounts of casein.
Electron micrographs revealed microvilli, tonofibrils and desmosomes.
Problematic cell line: Misidentified.
Presence of a Y chromosome in cell line that was thought to be of female origin (Yoshino et al.
2006.
Capes-Davies, 2010).
Originally thought to originate from a casein-producing breast cell line.
In addition contains SV40 genomic sequence while the cell line was deemed to be spontaneously immortalized.
Tumorigenic：Yes, in nude mice. At passage levels below 35 the line is not tumorigenic in nude mice, but forms colonies in soft agar. Tumorigenicity has been reported to increase above passage 35.
Viruses：The cells contain a tamdemly integrated SV40 genome it has been reported that they may contain a type D retrovirus that is similar or identical to Mason-Pfizer monkey virus (MPMV).
Reverse Transcriptase：Positive
Ploidy Status：Aneuploid
Msi Status：Stable (MSS)
Karyotype：The stemline chromosome number is near triploid with the modal number of 67 chromosomes, and the 2S component occurring at 0.6%. Most chromosome complements consist of about 39 normal and 28 marker chromosomes. Markers such as 2q, 11q+, 11q, t(2q.12), t(2q.5q?), t(6p?.16), 16pt and many others are common to most metaphases. Normal chromosomes 11, 14, 15 and 16 are absent. 2, 12, 17 and 19 are monosomic, and the x is disomic. DNA profiling for amelogenin, a sex-chromosome-specific PCR assay that can distinguish x chromosome-specific products from Y chromosome-specific products revealed the presence of Y chromosomes in this cell line of putative female origin. Confirmation of the general findings was accomplished by QM staining, C-banding, and FISH, with a whole chromosome paint probe to the human Y chromosome.
