83-5594-24　MCF-7 Cells 1vial　300273

特徴

• 概要：MCF7 cells, a staple in human breast cancer research, are utilized extensively as an in vitro model for hormone-dependent breast cancer.
• Originating from the breast tissue of a 69-year-old white female with metastatic adenocarcinoma, MCF 7 cells epitomize Luminal A subtype breast cancer, known for its non-invasive nature, poorly-aggressive behavior, and low metastatic potential, making them responsive to chemotherapy.
• MCF7 cells are adherent, with a population doubling time of approximately 24 hours, and possess a modal chromosome number of 82, though this can vary from 66 to 87.
• They retain key features of mammary epithelium but are characterized by low HER2 protein levels, contrasting with their expression of the WNT7B oncogene.
• In the realm of breast cancer research, MCF7 cells are instrumental in evaluating the efficacy of breast cancer drugs and understanding the dynamics of breast cancer stem cells.
• They are central to cancer research, serving as a comparative model against more aggressive cell lines like MDA-MB-231, elucidating the intricacies of carcinoma development.
• The growth of MCF7 cells is meticulously studied in both 2D cell culture and 3D cell culture systems, including spheroid culture, to mimic tumor microenvironments more closely.
• These methodologies enable a more profound exploration of cell spheroid growth and the behavior of cancer stem cells within microtissues in scaffold-based systems.
• The investigation of therapeutic agents, such as tamoxifen and doxorubicin, is critical in drug discovery efforts targeting hormone-dependent breast cancers.
• Tamoxifen, in particular, is scrutinized for its interaction with MCF7 breast cancer cells, providing valuable insights into the mechanisms of action and resistance.
• Similarly, the role of estradiol in modulating the growth and characteristics of these cells is a subject of significant interest, given its relevance to hormone-responsive breast cancers.
• Research employing the MCF7 breast cancer cell line often delves into the cellular processes of cytotoxicity and apoptosis, especially in response to cancer agents like curcumin, known for its potential in cancer prevention.
• The study of immune responses, including the action of tumor necrosis factor alpha (TNF alpha) and the impact of bacterial antigens, further enriches our understanding of the tumor microenvironment and potential therapeutic targets.
• Innovative approaches such as high voltage electrochemotherapy and the use of free hydrogels in 3D cultures are researched using MCF7 human breast cancer cells to enhance our understanding of drug delivery and efficacy.
• These studies provide highlight the challenges and difficulties encountered in treating hormone-dependent breast cancers.
• The MCF7 cell line, with its epithelial cell characteristics and resemblance to human adenocarcinoma cells, is a cornerstone of cancer research.
• It facilitates not only the exploration of breast cancer drugs and their mechanisms but also the broader implications for cancer treatment, including the potential role of mesenchymal stem cells and the efficacy of targeted therapies in vivo studies.