Karyotype:The karyotype is 45, xY, -7, -8, -17, -20, der(20)t(1,20)(q12,q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1,20)(q12,q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single x is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy.