T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM).
TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level).
TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
特徴
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).
The protein has a calculated MW of 41.1 kDa.
The protein migrates as 48-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：200ug
Source：Biotinylated Mouse TIGIT Protein, Fc,Avitag (MALS verified) (TIT-M82F3) is expressed from human 293 cells (HEK293). It contains AA Gly 26 - Thr 143 (Accession # A0A0B4J1G6-1).
Species Reactivity：Mouse
Host：HEK293
Tag：C-Fc & C-Avi
Exp Region：Gly 26 - Thr 143
Format：Powder
Conjugate：Biotin-labeled
Swiss-Prot：A0A0B4J1G6-1
Endotoxin：1.0 EU per μg
Purity：90%
Buffer：50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
