Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30).
IL-27 is produced by antigen-presenting cells.
IL-27 plays an important function in regulating the activity of B and T lymphocytes.
The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as IL27R and gp130.
IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells.
Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17).
It drives rapid clonal expansion of naive but not memory CD4 T-cells.
IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production.
IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells.
Another important role of IL-27 is its antitumor activity and antiangiogenic activity.
特徴
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 75.9 kDa.
The protein migrates as 44 kDa,48 kDa and 90-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：Human IL-27 Protein, Fc Tag (IL7-H5254) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Pro 243 & Arg 21 - Lys 229 (Accession # Q8NEV9-1 & Q14213-1).
Species Reactivity：Human
Host：HEK293
Tag：C-Fc
Exp Region：Phe 29 - Pro 243 & Arg 21 - Lys 229
Format：Powder
Conjugate：Unconjugated
Swiss-Prot：Q8NEV9-1 & Q14213-1
Endotoxin：1.0 EU per μg
Purity：85%
Buffer：51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5
