Human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity.
In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin.
Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1.
GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.
特徴
Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) and 81.8 kDa (ITGB4).
Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB4 contains no tag but a basic tail at the C-terminus.
The non-reducing (NR) protein migrates as 130-140 kDa (ITGA6) and 75-85 kDa (ITGB4) respectively due to glycosylation.
仕様
容量：1mg
Source：Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free (IN4-H52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 | Asn 28 - Ser 710 (Accession # P23229-2 & P16144-2).
Species Reactivity：Human
Host：HEK293
Tag：C-10×His | Native
Exp Region：Phe 24 - Gly 1012 | Asn 28 - Ser 710
Format：Powder
Conjugate：Unconjugated
Swiss-Prot：P23229-2 | P16144-2
Endotoxin：1.0 EU per μg
Purity：95%
Buffer：50 mM Tris,150 mM NaCl,0.2 M Arginine,pH7.5
