Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. GAD uses pyridoxal-phosphate (PLP) as a cofactor.Two molecular forms of GAD (65 kDa and 67 kDa) are highly conserved and both are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary.
The isoforms are regionally distributed cytoplasmically in the brains of rats and mice.
GAD65 is an ampiphilic, membrane-anchored protein (585 a.a.), and is responsible for vesicular GABA production.
GAD67 is cytoplasmic (594 a.a.), and seems to be responsible for significant cytoplasmic GABA production.
特徴
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 68.8 kDa.
The protein migrates as 66 kDa, 90 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：Human GAD1, His Tag (GA1-H5543) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Leu 594 (Accession # Q99259-1).
