Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated endonuclease from Lachnospiraceae bacterium，Cas12a, also known as Cpf1, is a 1200-1500 amino-acids long monomeric protein that belongs to the CRISPR/Cas system , an adaptive immune system of prokaryotes that has now become a powerful tool for genome editing.
CRISPR/Cas12a belongs the class II (type 5) of the CRISPR/Cas system that is defined by a single-subunit effector.
Cas12a has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity.
In addition, Cas12a is able to process more structured pre-CRISPR/RNA(crRNA) molecules into mature crRNAs which allows the possibility to use both mature or pre-crRNA for genome editing purposes.
All these features make the CRISPR/Cas12a system a valuable genome-engineering tool.
CRISPR-Cas12a has been successfully used to edit genomes in many mammalians cells and plants.
特徴
The protein has a calculated MW of 147.6 kDa.
The protein migrates as 135-145 kDa under reducing (R) condition (SDS-PAGE).
This protein has two NLS, which leads to transport to the nucleus efficiently.
It is ideal for in vitro and in vivo reactions.
仕様
容量：500ug
Source：GENPower NLS-Cas12a Nuclease (CAA-L5140) is expressed from E. coli cells. It contains AA Ser 2 - His 1228 (Accession # A0A5S8WF58).
Species Reactivity：Lachnospiraceae bacterium
Host：E. coli
Tag：N-10×His
Exp Region：Ser 2 - His 1228
Format：Liquid
Conjugate：Unconjugated
Swiss-Prot：A0A5S8WF58
Endotoxin：0.01 EU per μg
Purity：95%
Buffer：20 mM NaAC,500 mM NaCl,0.1 mM EDTA,0.1 mM TCEP,pH 6.0
