83-3877-99　Human LDL R Protein, Fc Tag （MALS verified） 100ug　LDR-H5251-100ug

特徴

• 背景
• Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL.
• It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.
• The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL).
• It belongs to the Low density lipoprotein receptor gene family.
• LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell.
• This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface.
• This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation.
• Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited.
• LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface.
• LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood.
• Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
• 特徴
• This protein carries a human IgG1 Fc tag at the C-terminus.
• The protein has a calculated MW of 111.2 kDa.
• As a result of glycosylation, the protein migrates as 120-125 kDa and 140-150 kDa under reducing (R) condition, and 270-330 kDa under non-reducing (NR) condition (SDS-PAGE).