83-3868-75　Ebolavirus EBOV （subtype Zaire, strain Kikwit-95） Envelope Glycoprotein （GP） Protein, His Tag 100ug　ZE5-V5221-100ug

特徴

• 背景
• EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L).
• GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP).
• GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment.
• Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly .
• GP1 is responsible for binding to the receptor(s) on target cells.
• Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell.
• GP2 acts as a class I viral fusion protein.
• GP1,2 mediates endothelial cell activation and decreases endothelial barrier function.
• sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
• 特徴
• Ebolavirus EBOV (subtype Zaire, strain Kikwit-95) GP, His Tag, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67.2 kDa.
• The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE.