It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2.
S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor.
S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
特徴
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.6 kDa.
The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：SARS-CoV-2 S2 protein (T716I, S982A, D1118H), His Tag (S2N-C52Hd) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein. The T716I/ S982A/ D1118H mutations were identified in the SARS-CoV-2 Alpha variant (Pango lineage: B.1.1.7; other names: 20I/501Y.V1 or VOC 202012/01).
