Nucleocapsid (N) protein is the most abundant protein found in coronavirus.
CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways.
It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M.
E., et al.
2017, Virology; 517: 62-68).
The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics.
Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
特徴
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 47.3 kDa.
The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：SARS-CoV-2 Nucleocapsid protein (T205I), His Tag (NUN-C52Hd) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (T205I)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation T205I was identified in the SARS-CoV-2 Beta (Pango Lineage: B.1.351) and Mu (Pango Lineage: B.1.621) variants (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).
