Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process.
C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond.
C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.
C5b has a transient binding site for C6.
The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor.
C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
特徴
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).
The protein has a calculated MW of 189.9 kDa.
As a result of glycosylation, the protein migrates as 75 kDa, 120 kDa and 200 kDa under reducing (R) condition, and 200 kDa under non-reducing (NR) condition (SDS-PAGE).
仕様
容量：25ug
Source：Biotinylated Human Complement C5, His,Avitag (CO5-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Cys 1676 (Accession # P01031-1).
