T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins.
CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex.
T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
特徴
Mouse CD3E&CD3D Heterodimer Protein, Fc, His Tag&Fc, Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 41.5 kDa (CD3E) and 40.7 kDa (CD3D).
Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus.
As a result of glycosylation, the heterodimer protein migrates as 55-65 kDa under reducing (R) condition, and 90-116 kDa under non-reducing (NR) condition (SDS-PAGE).
仕様
容量：1mg
Source：Mouse CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (CDD-M52W2) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).
Species Reactivity：Mouse
Host：HEK293
Tag：C-Fc & C-10×His | C-Fc & C-Flag
Exp Region：Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D)
Format：Powder
Conjugate：Unconjugated
Swiss-Prot：P22646-1 (CD3E) & P04235-1 (CD3D)
Protein ID：NP_031674.1 (CD3E) & NP_038515.3 (CD3D)
Endotoxin：1.0 EU per μg
Purity：95%
Buffer：50 mM Tris, 100mM Glycine, 25mM Arginine, 150mM NaCl, pH7.5
