Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family.
Cathepsin B / CTSB is synthesized as a preproenzyme.
Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme.
The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis.
Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.
特徴
This protein carries a polyhistidine tag at the C-terminus.
This protein contains an Activation peptide, and will be partially processed into Pro form with calculated MW of 37.3 kDa and mature form with calculated MW of 29 kDa under reducing (R) condition.
The protein migrates as 43-47 kDa and 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：1mg
Source：Mouse Cathepsin B, His Tag (CTB-M52H9) is expressed from human 293 cells (HEK293). It contains AA His 18 - Phe 339 (Accession # P10605-1).
