It is mainly synthesized by macrophages and liver.
C3 plays a central role in the activation of the complement system.
Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways.
It is cleaved into two fragments, C3a and C3b.
C3a anaphylatoxin is a mediator of local inflammatory process.
In chronic inflammation, acts as a chemoattractant for neutrophils.
After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
特徴
This protein carries a polyhistidine tag at the C-terminus.
The mature form of Complement component 3 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain.
Each α and β chain has a calculated MW of 71.3 kDa (β chain) and 113.0 kDa (α chain).
The protein migrates as 64-68 kDa (β chain) and 100-120 kDa (α chain) and 160-180 kDa (intact Complement component 3) under reducing (R) condition (SDS-PAGE) due to glycosylation.
仕様
容量：100ug
Source：Cynomolgus Complement C3, His Tag (CO3-C52H5) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asn 1663 (Accession # G7PYU9-1).
